Development and Validation of Sensitive, Fast and Simple LC-MS/MS Method to Investigate the Association between Adrenocortical Steroidogenesis and the High Intensity Exercise in Elite Athletes

The very high intensity of exercise accompanied by mental stress triggers adaptive mechanisms associated with adrenocortical steroidogenesis. However, the association between steroidogenesis and in elite athletes is poorly studied. A significant obstacle to solving this complex task wide range (4–5 orders) steroid concentrations serum limitations related amount biological samples taken from professional athletes. To solve task, we have developed validated a non-trivial approach for targeted metabolic profiling based on use LC-MS/MS dual-polarity electrospray ionization. method proposed allows quantitative determination 14 resistance biomarkers using small specimen within 8.5 min.